Scanning angle interference microscopy reveals cell dynamics at the nanoscale.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22751201.
- Also identified by DOI 10.1038/nmeth.2077 and PMC identifier 3454456.
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Abstract
Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton as well as the temporal evolution, three-dimensional architecture and nanoscale dynamics of focal adhesion complexes.
Medical subject headings
- Cell Membrane
- Cytoskeleton
- Epithelial Cells
- Microscopy, Interference
- Nanotechnology