Targeted gene knockout by direct delivery of zinc-finger nuclease proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22751204.
- Also identified by DOI 10.1038/nmeth.2030 and PMC identifier 3424280.
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Abstract
Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standard ZFN architecture and show that direct delivery of ZFNs as proteins leads to efficient endogenous gene disruption in various mammalian cell types with minimal off-target effects.
Medical subject headings
- Deoxyribonucleases, Type II Site-Specific
- Gene Knockout Techniques
- Genetic Engineering
- Recombinant Fusion Proteins
- Zinc Fingers