False discovery rate estimation for cross-linked peptides identified by mass spectrometry.
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- Record sourced from PubMed, PMID 22772729.
- Also identified by DOI 10.1038/nmeth.2103.
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Abstract
The mass spectrometric identification of chemically cross-linked peptides (CXMS) specifies spatial restraints of protein complexes; these values complement data obtained from common structure-determination techniques. Generic methods for determining false discovery rates of cross-linked peptide assignments are currently lacking, thus making data sets from CXMS studies inherently incomparable. Here we describe an automated target-decoy strategy and the software tool xProphet, which solve this problem for large multicomponent protein complexes.
Medical subject headings
- Cross-Linking Reagents
- Mass Spectrometry
- Peptides
- Proteomics