Multicolor two-photon tissue imaging by wavelength mixing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22772730.
- Also identified by DOI 10.1038/nmeth.2098.
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Abstract
We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.
Medical subject headings
- Color
- Microscopy, Fluorescence, Multiphoton
- Photons