In vitro gene amplification for prenatal diagnosis of congenital adrenal hyperplasia.

Rumsby, G; Honour, J W · J Med Genet · 1990

basic_science · Level V

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Abstract

A simple, rapid, non-radioactive method for detecting homozygous deletions/conversions of the steroid 21-hydroxylase gene is described. In our experience this method will be useful for first trimester prenatal diagnosis of congenital adrenal hyperplasia in 17% of families of a child with the salt losing form. This test includes an internal control to monitor the success of amplification.

Medical subject headings