Single-cell electroporation using a multifunctional pipette.

Ainla, Alar; Xu, Shijun; Sanchez, Nicolas; Jeffries, Gavin D M; Jesorka, Aldo · Lab Chip · 2012

basic_science · Level V

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Abstract

We present here a novel platform combination, using a multifunctional pipette to individually electroporate single-cells and to locally deliver an analyte, while in their culture environment. We demonstrate a method to fabricate low-resistance metallic electrodes into a PDMS pipette, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon microelectrode.

Medical subject headings