Direct observation of mammalian cell growth and size regulation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22863882.
- Also identified by DOI 10.1038/nmeth.2133 and PMC identifier 3433595.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
Medical subject headings
- Cell Enlargement
- Cell Size
- Lymphocytes
- Microfluidic Analytical Techniques
- Precursor Cells, B-Lymphoid
- Single-Cell Analysis