Tomography and static-mechanical properties of adherent cells.

Soh, Siowling; Kandere-Grzybowska, Kristiana; Mahmud, Goher; Huda, Sabil; Patashinski, Alexander Z; Grzybowski, Bartosz A · Adv Mater · 2012

basic_science · Level V

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Abstract

A tomography approach is used to reconstruct 3D cell shapes and, simultaneously, the shapes/positions of the nuclei within these cells. Subjecting the cells to well-defined microconfinements of various diameters allow for relating the steady-state shapes of cells to their static-mechanical properties. The observed shapes show striking regularities between different cell types and all fit to a model that takes into account the cell membrane, cortical actin, and the nucleus.

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