A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.
basic_science · Level V
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Abstract
Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.
Medical subject headings
- Microfluidic Analytical Techniques
- Models, Biological
- Neutrophils
- Transendothelial and Transepithelial Migration