A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA).
basic_science · Level V
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- Record sourced from PubMed, PMID 22914859.
- Also identified by DOI 10.1039/c2lc40585g.
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Abstract
In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.
Medical subject headings
- Enzyme-Linked Immunosorbent Assay
- Microfluidic Analytical Techniques