Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
case_report · Level V
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- Record sourced from PubMed, PMID 22937078.
- Also identified by DOI 10.1371/journal.pone.0043682 and PMC identifier PMC128099.
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Abstract
Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.