Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22941364.
- Also identified by DOI 10.1038/nmeth.2177 and PMC identifier 3602999.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.
Medical subject headings
- DNA Breaks, Double-Stranded
- Endonucleases