Replication of hepatitis C virus genotype 3a in cultured cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22999961.
- Also identified by DOI 10.1053/j.gastro.2012.09.017.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Hepatitis C virus (HCV) genotype 3a is widespread worldwide, but no replication system exists for its study. We describe a subgenomic replicon system for HCV genotype 3a. We determined the consensus sequence of an HCV genome isolated from a patient, and constructed a subgenomic replicon using this clone. The replicon was transfected into HuH-7 cells and RNA replication was confirmed. We identified cell culture-adaptive mutations that increased colony formation multiple-fold. We have therefore established a genotype 3a replicon system that can be used to study this HCV genotype.
Medical subject headings
- Hepacivirus
- RNA, Viral
- Replicon
- Virus Replication