Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts.
basic_science · Level V
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- Record sourced from PubMed, PMID 2300565.
- Also identified by PMC identifier 53357.
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Abstract
We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.
Medical subject headings
- Cloning, Molecular
- Genes
- Photosynthesis
- Plants
- Transfection