Serum immunoreactive inhibin levels before and after luteectomy in the cynomolgus monkey (Macaca fascicularis).

Abstract

Whereas studies in women have demonstrated that serum immunoreactive inhibin concentrations peak during the luteal phase of the menstrual cycle and that the corpus luteum (CL) encodes mRNA for the inhibin subunits, a clear link between the presence of the CL and circulating inhibin has not been established in primates. Therefore, we measured serum immunoreactive inhibin levels in monkeys before and after luteectomy as well as immunoreactive inhibin concentrations and mRNA encoding the alpha-inhibin subunit in luteal tissue. Monkeys were assigned to one of four groups depending on which day of the luteal phase luteectomy was performed: group A, days 4-5; group B, days 7-8; group C, days 9-10; and group D, days 11-12 (the day after the estrogen surge = day 1 of the luteal phase). Daily blood samples were obtained for 3 days before luteectomy, immediately before surgery, and for 2 days after luteectomy. Immunoreactive inhibin concentrations were measured with a double antibody RIA using an antiserum to bovine 31-kDa inhibin, bovine 31-kDa inhibin for iodination, and a human follicular fluid inhibin preparation as standard. Total RNA was isolated from luteal tissue and transferred by Northern blot onto a Zeta-probe membrane. The probe used for hybridization was the PstI/NcoI restriction enzyme fragment (381 basepairs) of alpha-inhibin DNA generated from a human ovarian cDNA library. Serum inhibin concentrations decreased (P less than 0.05) 24 h after removal of the corpus luteum in each of the four groups studied.(ABSTRACT TRUNCATED AT 250 WORDS)