Staining and embedding the whole mouse brain for electron microscopy.
basic_science · Level V
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- Record sourced from PubMed, PMID 23085613.
- Also identified by DOI 10.1038/nmeth.2213.
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Abstract
The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single-axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block-face electron microscopy.
Medical subject headings
- Brain
- Microscopy, Electron
- Staining and Labeling
- Tissue Embedding