Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23085614.
- Also identified by DOI 10.1038/nmeth.2211 and PMC identifier 3703907.
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Abstract
We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.
Medical subject headings
- Membrane Proteins
- Metal Nanoparticles
- Protein Binding