Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23093669.
- Also identified by DOI 10.1073/pnas.1216473109 and PMC identifier 3511126.
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Abstract
We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome. This procedure increased transgene integration efficiency by fivefold compared with conventional PNI or intracytoplasmic sperm injection-mediated transgenesis. Our data indicate that the transposase-enhanced PNI technique additionally requires fewer embryos to be microinjected than traditional methods to obtain transgenic animals. This transposase-mediated approach is also very efficient for single-cell embryo cytoplasmic injections, offering an easy-to-implement transgenesis method to the scientific community.
Medical subject headings
- Cell Nucleus
- DNA Transposable Elements
- Gene Transfer Techniques
- Microinjections
- Transposases