Phase-gradient microscopy in thick tissue with oblique back-illumination.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23103879.
- Also identified by DOI 10.1038/nmeth.2219 and PMC identifier 8407656.
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Abstract
Phase-contrast techniques, such as differential interference contrast microscopy, are widely used to obtain morphological images of unstained biological samples. The transillumination geometry required for these techniques restricts their application to thin samples. We introduce oblique back-illumination microscopy, a method of collecting en face phase-gradient images of thick scattering samples, enabling near-video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy.
Medical subject headings
- Microscopy