Ultrabright photoactivatable fluorophores created by reductive caging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23103881.
- Also identified by DOI 10.1038/nmeth.2214 and PMC identifier 3561463.
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Abstract
Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)-10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum.
Medical subject headings
- Carbocyanines
- Fluorescent Dyes
- Microscopy, Fluorescence