Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23132304.
- Also identified by DOI 10.1039/c2lc40948h.
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Abstract
Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.
Medical subject headings
- Cytochrome P-450 CYP3A
- Drug Evaluation, Preclinical
- Luminescent Measurements
- Microarray Analysis