GPR39 is coupled to TMEM16A in intestinal fibroblast-like cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23133519.
- Also identified by DOI 10.1371/journal.pone.0047686 and PMC identifier 3485058.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
GPR39 is a GPCR implicated as a regulator of gastrointestinal motility, although the mechanism remains elusive. Here, we report that GPR39 is expressed by a specific cell population cultured from mouse small intestine muscle layers, which was subsequently identified as fibroblast-like cells (FLCs) that have recently been shown to modulate gut motility. Application of the GPR39 agonist, Zn(2+), induced large currents and membrane depolarization in FLCs cultured from wild-type mice, but not Gpr39(-/-) mice. This Zn(2+)-induced current could be suppressed by application of a TMEM16A antagonist, CaCC(inh)-A01, or by silencing Tmem16a expression. These data suggest that GPR39 might modulate gut motility via regulating TMEM16A function in FLCs.
Medical subject headings
- Chloride Channels
- Fibroblasts
- Gastrointestinal Tract
- Gene Expression Regulation
- Receptors, G-Protein-Coupled