Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23142872.
- Also identified by DOI 10.1038/nmeth.2225 and PMC identifier 3863600.
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Abstract
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
Medical subject headings
- Microfluidics
- Nucleotide Motifs
- RNA