Two-photon optogenetics of dendritic spines and neural circuits.
basic_science · Level V
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- Record sourced from PubMed, PMID 23142873.
- Also identified by DOI 10.1038/nmeth.2249 and PMC identifier 3518588.
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Abstract
We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.
Medical subject headings
- Dendritic Spines
- Neurons
- Photons