Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
basic_science · Level V
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- Record sourced from PubMed, PMID 23144871.
- Also identified by DOI 10.1371/journal.pone.0048381 and PMC identifier PMC3105465.
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Abstract
Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.