Measuring uptake dynamics of multiple identifiable carbon nanotube species via high-speed confocal Raman imaging of live cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23151070.
- Also identified by DOI 10.1021/nl302991y and PMC identifier 3561480.
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Abstract
Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic and nine nanotube-derived Raman bands was conducted simultaneously in RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, and aggregation states of nanotubes transiently as well as the cell position, denoted by lipid and protein signals. This work portends the real-time molecular imaging of live cells and tissues using Raman spectroscopy, affording multiplexing and complete photostability.
Medical subject headings
- Macrophages
- Microscopy, Confocal
- Nanotubes, Carbon
- Spectrum Analysis, Raman