Numerical and experimental evaluation of platelet deposition to collagen coated surface at low shear rates.

Abstract

Platelet deposition to collagen-coated surface under low shear conditions was investigated using an experimental model. The flow chamber was created by combining a stationary and a rotational glass plates spaced 50 μm apart. Blood filled into this space was subjected to a simple Couette flow. Both glass plates were covered with albumin to render them anti-thrombogenic. However, one spot 1×1 mm in size was covered with collagen. This spot was where the platelets deposited. The device was mounted on an inverted microscope and the platelet deposition was recorded. Platelets were dyed to render them fluorescent. The blood used was human blood from healthy volunteers. It was subjected to a range of low shear rates (below 7001/s) to find out how they act on platelet deposition. The results show a characteristic curve with elevated platelet deposition in the range of 1501/s. For the interpretation of these results a numerical model was developed. It applies the Monte Carlo method to model a random walk of platelets. This diffusive motion was superimposed on the convective motion by the Couette flow. A satisfactory match to the experimental data was achieved.