Direct imaging of DNA fibers: the visage of double helix.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23171353.
- Also identified by DOI 10.1021/nl3039162.
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Abstract
Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.
Medical subject headings
- Bacteriophage lambda
- DNA, Viral