Enhanced enzymatic thermal stability and activity in functionalized mesoporous silica monitored by (31) p NMR.

El-Boubbou, Kheireddine; Schofield, David A; Landry, Christopher C · Adv Healthc Mater · 2012

basic_science · Level V

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Abstract

Organophosphorus hydrolase (OPH) is immobilized on ammonium-modified mesoporous silica particles. Thermal stability and activity are measured with a (31) P NMR assay of the conversion of paraoxon (toxic) to its non-toxic hydrolysis product. After immobilization, OPH is significantly more active at room temperature and retained activity even after being heated to 45 °C for 1 month.

Medical subject headings