Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23223154.
- Also identified by DOI 10.1038/nmeth.2277 and PMC identifier 4161287.
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Abstract
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
Medical subject headings
- Caenorhabditis elegans
- Cell Tracking
- Imaging, Three-Dimensional
- Microscopy, Fluorescence
- Neurons
- Saccharomyces cerevisiae