Optogenetic control of transcription in zebrafish.
basic_science · Level V
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- Record sourced from PubMed, PMID 23226369.
- Also identified by DOI 10.1371/journal.pone.0050738 and PMC identifier PMC3435206.
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Abstract
Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.