In vitro cultivation of the hymenoptera genetic model, Nasonia.
basic_science · Level V
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- Record sourced from PubMed, PMID 23227258.
- Also identified by DOI 10.1371/journal.pone.0051269 and PMC identifier PMC2916728.
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Abstract
The wasp genus Nasonia is a genetic model with unique advantages for the study of interspecific differences, including haplodiploidy and interfertile species. However, as a parasitoid, Nasonia is confined within a fly host, thus restricting direct observations and manipulation of development over time. Here, we present the first in vitro cultivation method for this system that decouples Nasonia from its host, allowing continuous observations from embryo to adulthood. Using transwell plates and a simple Nasonia rearing medium, we demonstrate a technique that will significantly expand the utility of the Nasonia model.