Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity.
basic_science · Level V
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- Record sourced from PubMed, PMID 23236516.
- Also identified by DOI 10.1371/journal.pone.0051559 and PMC identifier PMC2708616.
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Abstract
Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems. Here we used different vaccination schemes in order to systematically compare virus-like particle preparations generated in the two systems. Our work shows significant differences in immunogenicity between the two, and indicates superior and broader immune responses induced by the baculovirus-derived constructs. We demonstrate that these differences critically influence protection and survival in a mouse model of influenza virus infection. Finally, we show that the enhanced immunogenicity of the baculovirus-derived virus-like particles is caused by contamination with residual baculovirus which activates the innate immune response at the site of inoculation.