Quantitative optical trapping on single organelles in cell extract.
basic_science · Level V
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- Record sourced from PubMed, PMID 23241632.
- Also identified by DOI 10.1038/nmeth.2287 and PMC identifier 3605797.
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Abstract
We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.
Medical subject headings
- Kinesins
- Lipid Metabolism
- Lipids
- Optical Tweezers
- Organelles