Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23263690.
- Also identified by DOI 10.1038/nmeth.2301 and PMC identifier 3605914.
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Abstract
We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.
Medical subject headings
- Genome, Protozoan
- Toxoplasma