A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23263692.
- Also identified by DOI 10.1038/nmeth.2306 and PMC identifier 3735345.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.
Medical subject headings
- DNA Primers
- Genomic Library
- In Situ Hybridization, Fluorescence
- Sequence Analysis, DNA