Dual-wavelength fluorescent detection of particles on a novel microfluidic chip.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23291857.
- Also identified by DOI 10.1039/c2lc41238a.
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Abstract
This paper reports a novel lab-on-a-chip device that performs fluorescent particle counting by coupling the electrokinetically-induced pressure-driven flow and a miniaturized dual-wavelength fluorescent detection method. A novel L-shaped PDMS microchannel bonded on a thin glass slide is used to transport the particles. The dual-wavelength fluorescent detection system can count two different fluorescent particles simultaneously. Good agreement is achieved between the results obtained by the microfluidic chip device and the results from a commercial flow cytometer.
Medical subject headings
- Fluorescent Dyes
- Microfluidic Analytical Techniques