Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes.

Schirwitz, Christopher; Loeffler, Felix F; Felgenhauer, Thomas; Stadler, Volker; Nesterov-Mueller, Alexander; Dahint, Reiner; Breitling, Frank; Bischoff, F Ralf · Adv Mater · 2013

basic_science · Level V

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Record sourced from PubMed, PMID 23315653.
Also identified by DOI 10.1002/adma.201203853.
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Abstract

A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.

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