rsEGFP2 enables fast RESOLFT nanoscopy of living cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 23330067.
- Also identified by DOI 10.7554/eLife.00248 and PMC identifier 3534202.
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Abstract
The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:http://dx.doi.org/10.7554/eLife.00248.001.
Medical subject headings
- Epithelial Cells
- Green Fluorescent Proteins
- Microscopy, Fluorescence