A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets.
basic_science · Level V
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- Record sourced from PubMed, PMID 23334102.
- Also identified by DOI 10.1038/nmeth.2341.
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Abstract
We introduce a biophysical model of miRNA-target interaction and infer its parameters from Argonaute 2 cross-linking and immunoprecipitation data. We show that a substantial fraction of human miRNA target sites are noncanonical and that predicted target-site affinity correlates well with the extent of target destabilization. Our model provides a rigorous biophysical approach to miRNA target identification beyond ad hoc miRNA seed-based methods.
Medical subject headings
- Argonaute Proteins
- Biophysical Phenomena
- Gene Targeting
- MicroRNAs
- Models, Biological
- RNA, Messenger