Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23360306.
- Also identified by DOI 10.1021/nl304359p and PMC identifier 3696529.
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Abstract
The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60° can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.
Medical subject headings
- Microscopy, Fluorescence
- Nanotechnology