Efficient genome editing in zebrafish using a CRISPR-Cas system.
basic_science · Level V
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- Record sourced from PubMed, PMID 23360964.
- Also identified by DOI 10.1038/nbt.2501 and PMC identifier 3686313.
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Abstract
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
Medical subject headings
- Genome
- Inverted Repeat Sequences
- Zebrafish