Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23360966.
- Also identified by DOI 10.1038/nbt.2507.
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Abstract
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
Medical subject headings
- Endonucleases
- Genetic Engineering
- Genome, Human