Ultrafast cell switching for recording cell surface transitions: new insights into epidermal growth factor receptor signalling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23385220.
- Also identified by DOI 10.1039/c3lc41297k.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A pinched-flow deflection technology was developed for rapid single cell switching between biochemical microenvironments. Millisecond switching was used to stimulate and preserve epidermal growth factor receptor (EGFR) autophosphorylation transitions. Intramolecular phosphorylation initiates signal transduction, is silenced by phosphatase activity until EGFR dimerization enables intermolecular phosphorylation to initiate downstream signalling.
Medical subject headings
- ErbB Receptors
- Signal Transduction