Robust, synergistic regulation of human gene expression using TALE activators.
basic_science · Level V
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- Record sourced from PubMed, PMID 23396285.
- Also identified by DOI 10.1038/nmeth.2366 and PMC identifier 3584229.
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Abstract
Artificial activators designed using transcription activator-like effector (TALE) technology have broad utility, but previous studies suggest that these monomeric proteins often exhibit low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy, and guide design of monomeric TALE-based fusion proteins.
Medical subject headings
- Genetic Engineering
- MicroRNAs
- Transcription Factors
- Transcriptional Activation
- Vascular Endothelial Growth Factor A