Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation.
basic_science · Level V
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- Record sourced from PubMed, PMID 23416756.
- Also identified by DOI 10.1038/nmeth.2372 and PMC identifier 4131260.
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Abstract
We report intron chromosomal expression FISH (iceFISH), a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We find substantial differences in transcriptional frequency between genes on a translocated chromosome and the same genes in their normal chromosomal context in the same cell. Correlations between genes on a single chromosome pointed toward a cis chromosome-level transcriptional interaction spanning 14.3 megabases.
Medical subject headings
- Chromosomes, Human, Pair 13
- Chromosomes, Human, Pair 19
- Gene Expression Profiling
- Gene Expression Regulation
- Introns