Neutron-encoded mass signatures for multiplexed proteome quantification.
basic_science · Level V
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- Record sourced from PubMed, PMID 23435260.
- Also identified by DOI 10.1038/nmeth.2378 and PMC identifier 3612390.
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Abstract
We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.
Medical subject headings
- Chromatography, Liquid
- Neutrons
- Proteomics
- Saccharomyces cerevisiae Proteins
- Tandem Mass Spectrometry