Micro-optical lens array for fluorescence detection in droplet-based microfluidics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23455606.
- Also identified by DOI 10.1039/c3lc41329b and PMC identifier 3697795.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals. We show that the integration of microlenses and mirror surfaces in these devices results in an 8-fold increase in the fluorescence signal and in improved spatial resolution. Using an array of microlenses, massively parallel detection of droplets containing fluorescent dyes was achieved, leading to detection throughputs of about 2000 droplets per second and per lens, parallelized over 625 measurement points.
Medical subject headings
- Fluorescent Dyes
- Microfluidic Analytical Techniques
- Optical Devices