QuaNCAT: quantitating proteome dynamics in primary cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 23474466.
- Also identified by DOI 10.1038/nmeth.2401 and PMC identifier 3676679.
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Abstract
Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
Medical subject headings
- CD4-Positive T-Lymphocytes
- Gene Expression Regulation
- Proteomics