Fast spore breaking by superheating.

Přibylka, Adam; Almeida, Ana Vanessa; Altmeyer, Matthias O; Petr, Jan; Sevčík, Juraj; Manz, Andreas; Neužil, Pavel · Lab Chip · 2013

basic_science · Level V

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Abstract

Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 °C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 °C resulted in the release of DNA within 20 s.

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